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Hydrolyzed Hyaluronic Acid

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General Information

Product Name: Hydrolyzed Hyaluronic Acid
Catalog: CI-HA-010
Applications: Suitable in anti-aging, moisturizing and repairing skin care products

Hydrolyzed hyaluronic acid is a safe, non-irritating moisturizer that has undergone a process of hydrolysis in which one of the components is converted from one form to another by the addition of water or enzymes. As a result, it produces hyaluronic acid with a lower molecular weight, which means it penetrates the skin more easily (pure hyaluronic acid is a large molecule). While this does not mean that hydrolyzed hyaluronic acid is better than non-hydrolyzed hyaluronic acid, it works in a different way. The hydrolyzed hyaluronic acid we offer has a molecular weight of 37 kDa – 56 kDa. It has the ability to reduce wrinkles, to improve skin elasticity, hydration and to decrease transepidermal water loss (TEWL). It also can increase collagen production, reduce inflammation and improve wound healing.

Alfa Chemistry can precisely control the molecular weight range of hyaluronic acid and provide sodium hyaluronate in different molecular weight segments. We can also provide comprehensive technical support from product use to efficacy evaluation.

Items	Specification
Appearance	Powder or granules
Recommended dosage	0.1%-0.5%
Functions	Reducing wrinkles, improving skin elasticity, improving hydration and decreasing trans epidermal water loss
Heavy metals	≤ 20ppm
Arsenic	≤ 2ppm
Bacteria count	≤ 100cfu/g
Escherichia coli	Negative
Staphylococcus aureus	Negative
Pseudomonas aeruginosa	Negative
Storage	Lightproof, ordinary temperature, and sealed container
Validity	Two years

As a leading provider of cosmetic raw materials, Alfa Chemistry can supply high-quality hydrolyzed hyaluronic acid to cosmetic companies looking to create innovative skincare products. For high quality products and more information, please feel free to contact us.

Case Study

Hydrolyzed Hyaluronic Acid for Enhancing Skin Barrier Function and Hydration

Effects of topical application of LMHA on filaggrin formation and enzymatic turnover in a 3D epidermal model. Hashimoto, Moe, et al. Cosmetics 8.4 (2021): 118.

In this study, topical low-molecular-weight (LM) hydrolyzed hyaluronic acid (LMHA; HA fragments generated by chemical or enzymatic cleavage, also known as hydrolyzed hyaluronic acid) applied on in vitro three-dimensional (3D) human epidermis to evaluate its potential to support the natural skin barrier by driving the production and subsequent processing of the barrier protein filaggrin (FLG), the precursor to natural moisturizing factors (NMF).

Driving the Production of the Skin Barrier Protein Filaggrin: Topical application of LMHA to the 3D epidermal model enhanced mRNA expression of the two main differentiation markers profilaggrin (proFLG) and involucrin (IVL). This result was validated at protein level, with Western blotting and immunofluorescence staining revealing the topical application of LMHA drove expression of FLG and IVL proteins.
Driving Production of NMF: The study provides evidence LMHA exerts an active impact on the enzymatic cascade that drives the generation of NMF. Application of the LMHA resulted in an increase in mRNA expression of caspase-14 (CASP14) and bleomycin hydrolase (BLMH), the enzymes responsible for the proteolytic cleavage of FLG to amino acids that together make up the NMF. In addition, the treatment directly and dose-dependently increased the enzymatic activity of CASP14 in the stratum granulosum (SG) and stratum corneum (SC) of the 3D model.
Support of Epidermal Differentiation and Barrier Integrity: Immunofluorescence assessment also revealed treatment of 3D skin with LMHA resulted in a more intense fluorescence signal for the FLG protein compared to the untreated control model, indicating superior epidermal differentiation and a concomitant lower degree of incomplete keratinization.

Hydrolyzed Hyaluronic Acid (2k Da) as an Active Ingredient for Collagen Remodeling and Wrinkle Amelioration

The skin lotion formula based on hydrolyzed hyaluronic acid and the skin permeation images of hydrolyzed HA. Abe, Yukina, et al. Journal of Cosmetic Dermatology 22.1 (2023): 320-327.

This study focused on a specific fraction of hydrolyzed hyaluronic acid, defined as 2-kDa HA oligosaccharides (HA2k). Topical formulation containing 0.1% 2-kDa hyaluronan (HA2k), formulated as a lotion and applied in human forearm and facial (nasolabial, crow's-feet) studies. Preclinical assays used fluorescein-labeled HA2k in 3D epidermal models and excised human skin. No adverse inflammatory effects were observed in the clinical study.

Epidermal Permeation: Fluorescent microscopy confirmed that HA2k penetrated through the stratum corneum in both 3D epidermal models and excised human skin, indicating the oligosaccharide reaches deeper skin layers relevant to dermal remodeling.
Collagen Metabolism Modulation: In the 3D skin model, topical HA2k application increased levels of both COL1A1 and MMP-1, demonstrating simultaneous activation of collagen synthesis and collagen degradation pathways consistent with a remodeling process rather than unidirectional accumulation.
Increased Dermal Collagen Density: In the human forearm study, treatment with the 0.1% HA2k solution resulted in a measurable increase in dermal collagen density compared to the vehicle control site after 4 weeks of application.
Reduction in Wrinkle Depth: The randomized controlled facial trial demonstrated a statistically significant reduction in the maximum depth of wrinkles in both the nasolabial fold and crow's feet areas for the group using the 0.1% HA2k lotion compared to the control group after 8 weeks.

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